RNA-Seq data (PRJNA490626) were downloaded from the website (http://grand.cricaas.com.cn/page/tools/expressionVisualization ) to examine the relative expression patterns of 2ODDs under abiotic stress (cold, heat, salt and PEG) with various time periods (0, 1, 3, 6 and 12 for each treatment) and different tissues including the root, shoot, petal, leaf, pistil, stamen, torus and calycle, along with the ovule and fiber [78 (link)]. Zhang's transcriptome data under different saline-alkali stress (100 mM NaCl, 50 mM Na2CO3, 0.125 mM NaOH) were used to determine the expression levels of Gh2ODD family members [79 ]. The heat map was generated through the TBtools software with FPKM values for relative expression analysis.
G. hirsutum accession Zhong 9807 was used in this study. Cotton seedlings at three true leaf stage under normal field conditions were treated by various abiotic stresses and sampled for tissue specific expression analysis. Tissue specific expression profilings of different Gh2ODDs in the root, stem, leaf, petal, stamen, pistil, ovule and fiber were assayed. The expression of different Gh2ODDs in cotton response to salt (400 mM NaCl), drought (20% PEG), cold (4℃) and heat (37℃) for different time points (1, 3, 6 and 12 h) were analyzed. We used the FastPure® Plant Total RNA lsolation Kit (Nanjing Vazyme Biotech Co., Ltd.) to extract total RNA from all samples of cotton plants which were further used to synthesize cDNA with Prime-Script®RT reagent kit (Takara, China) following manual instruction. All the samples were collected and transferred to liquid nitrogen immediately, saved at – 80℃ for future use.
G. hirsutum accession Zhong 9807 was used in this study. Cotton seedlings at three true leaf stage under normal field conditions were treated by various abiotic stresses and sampled for tissue specific expression analysis. Tissue specific expression profilings of different Gh2ODDs in the root, stem, leaf, petal, stamen, pistil, ovule and fiber were assayed. The expression of different Gh2ODDs in cotton response to salt (400 mM NaCl), drought (20% PEG), cold (4℃) and heat (37℃) for different time points (1, 3, 6 and 12 h) were analyzed. We used the FastPure® Plant Total RNA lsolation Kit (Nanjing Vazyme Biotech Co., Ltd.) to extract total RNA from all samples of cotton plants which were further used to synthesize cDNA with Prime-Script®RT reagent kit (Takara, China) following manual instruction. All the samples were collected and transferred to liquid nitrogen immediately, saved at – 80℃ for future use.
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