Immunohistochemical Analysis of Substantia Nigra

After 3 months of WGE treatment, mice were sacrificed at the age of 11.5 months. The substantia nigra and striatum were dissected out. The substantia nigra was subjected to immunostaining, as described previously (Lin et al., 2020 (link)). Anti-tyrosine hydroxylase (TH) (Millipore, AB152, 1:200) and anti-ionized calcium-binding adapter molecule 1 (Iba-1) (GeneTex, GTX100042, 1:200) were used as primary antibodies for 24 hr at 4°C. Secondary antibodies were DyLight 488 goat anti-rabbit 1:300 and Alexa Fluor 546 goat anti-rabbit at 1:200 (25°C for 1 hr). Mounting medium with DAPI (GeneTex, GTX30920) was used as a counterstain.

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Lin Y.E., Lin C.H., Ho E.P., Ke Y.C., Petridi S., Elliott C.J., Sheen L.Y, & Chien C.T. (2021). Glial Nrf2 signaling mediates the neuroprotection exerted by Gastrodia elata Blume in Lrrk2-G2019S Parkinson’s disease. eLife, 10, e73753.

Publication 2021

AB152 GTX100042 GTX30920

After treatment Alexa fluor 546 Antibodies Calcium Dapi Goat Mice Rabbit Striatum Substantia nigra Tyrosine hydroxylase

Corresponding Organization :

Other organizations : National Taiwan University, Institute of Molecular Biology, Academia Sinica, National Taiwan University Hospital, University of York, MRC Mitochondrial Biology Unit, University of Cambridge, Academia Sinica

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Variable analysis

independent variables

WGE treatment

dependent variables

Tyrosine hydroxylase (TH) expression
Ionized calcium-binding adapter molecule 1 (Iba-1) expression

control variables

Age of mice at 11.5 months
Dissection of substantia nigra and striatum

controls

Positive control: Not specified
Negative control: Not specified

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