Neuroblastoma Cell Neurite Outgrowth Assay

Neuroblastoma cells were cultured in standard media or with UAB30 (10 µM, 48 hours). Pictures [Photometrics CoolSNAP HQ2 CCD camera (Tucson, AZ) attached to a Nikon Eclipse Ti microscope (Tokyo, Japan)] were obtained and the number of neurite outgrowths per cell were counted and reported as fold change neurite outgrowths [11 (link)].

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Waters A.M., Stewart J.E., Atigadda V.R., Mroczek-Musulman E., Muccio D.D., Grubbs C.J, & Beierle E.A. (2015). Pre-Clinical Evaluation of a Novel RXR Agonist for the Treatment of Neuroblastoma. Molecular cancer therapeutics, 14(7), 1559-1569.

Publication 2015

CoolSNAP HQ2 CCD camera Eclipse Ti microscope

Cell Cultured media Microscope Neurite outgrowths Neuroblastoma

Corresponding Organization :

Other organizations : University of Alabama at Birmingham

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Variable analysis

independent variables

Culture media (standard media or with UAB30 (10 µM))

dependent variables

Number of neurite outgrowths per cell (reported as fold change)

control variables

Culture duration (48 hours)

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