Wild type mice (C57/B6), SOD1 G93A transgenic mice, BAC GLT1 eGFP (previously generated in our lab) (Regan et al. 2007 (link)), BAC NG2 DsRed (kind gift of Dr. Dwight Bergles, Dept. of Neuroscience, Johns Hopkins University), BAC ALDH1L1 eGFP mice (GENSAT project), GFAP eGFP mice (Jackson laboratory) were used for in vivo experiments. The care and treatment of animals in all procedures strictly followed the NIH Guide for the Care and Use of Laboratory Animals and the Guidelines for the Use of Animals in Neuroscience Research and the Johns Hopkins University IACUC. Mice were housed at standard temperature (21°C) and in a light controlled environment with ad libitum access to the food and water. BAC ALDH1L1 eGFP (and GFAP eGFP) mice were crossed with SOD1 G93A mice to obtain double transgenic mice. Littermates were used as control. Mice were sacrificed at designated time point following breeding or experiment. A 30-gauge needle was used to induce an acute lesion in the striatum.