Co-immunoprecipitation of β-actin and V5-PFN1

Co-immunoprecipitation of ß-actin with V5-PFN1 in lysates of HEK293 cells was performed as previously described [2 (link)]. For Western blot detection of V5-PFN1 and ß-actin we used mouse anti-V5 (Life Technologies Cat# R96025 RRID:AB_10050879) and rabbit anti-ß-actin (Cell Signaling Technology Cat# 4970 RRID:AB_2223172) antibodies. Secondary antibodies were goat anti-mouse IgG-HRP (Life Technologies Cat# G21040 RRID:AB_11180340) and goat anti-rabbit IgG-HRP (Life Technologies Cat# G21234 RRID:AB_10837906), respectively.

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Freischmidt A., Schöpflin M., Feiler M.S., Fleck A.K., Ludolph A.C, & Weishaupt J.H. (2015). Profilin 1 with the amyotrophic lateral sclerosis associated mutation T109M displays unaltered actin binding and does not affect the actin cytoskeleton. BMC Neuroscience, 16, 77.

Publication 2015

mouse anti-V5 rabbit anti-ß-actin goat anti-mouse IgG-HRP goat anti-rabbit IgG-HRP

Actin Anti igg Antibodies Co immunoprecipitation Goat Hek293 cells Mouse Rabbit Western blot

Corresponding Organization :

Other organizations : Universität Ulm

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Variable analysis

independent variables

Presence of V5-PFN1 in HEK293 cell lysates

dependent variables

Co-immunoprecipitation of β-actin with V5-PFN1

control variables

Lysates of HEK293 cells

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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