Quantification of Lung Cell Chemokines

Mouse lung epithelial cells (MLE12 cells) were obtained from ATCC (Manassas, VA, USA). Real-time PCR was performed using an iCycler™ (Bio-Rad, Hercules, California, USA) to determine the levels of CCL17 (thymus and activation-regulated chemokine, TARC), CCL22 (macrophage-derived chemokine, MDC), CCL11 (eotaxin), thymic stromal lymphopoietin (TSLP), and IL-25 mRNA. GAPDH was used as an internal reference. Primers and PCR conditions have been described previously [30] (link)–[32] (link).

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Park M.K., Cho M.K., Kang S.A., Park H.K., Kim D.H, & Yu H.S. (2014). Acanthamoeba Protease Activity Promotes Allergic Airway Inflammation via Protease-Activated Receptor 2. PLoS ONE, 9(3), e92726.

Publication 2014

MLE12 cells iCycler

Ccl17 Ccl22 Chemokine Eotaxin Epithelial cells Gapdh Il 25 Lung Macrophage derived chemokine Mouse Mrna Primers Real time pcr Thymic stromal lymphopoietin Thymus

Corresponding Organization :

Other organizations : Pusan National University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of CCL17 (thymus and activation-regulated chemokine, TARC) mRNA
Levels of CCL22 (macrophage-derived chemokine, MDC) mRNA
Levels of CCL11 (eotaxin) mRNA
Levels of thymic stromal lymphopoietin (TSLP) mRNA
Levels of IL-25 mRNA

control variables

GAPDH as an internal reference

controls

No positive or negative controls were explicitly mentioned in the provided information.

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