Isolation of Genetically Modified Aortic Endothelial Cells
Corresponding Organization :
Other organizations : Nanjing Medical University, University of California, Davis
Variable analysis
- Genotype of mice (Rptor^EC-/-, Rictor^EC-/-, Mtor^EC-/- or wild-type littermates)
- Purity of endothelial cells (EC) isolated from mouse aorta, examined by the uptake of Dil-Ac-LDL and FITC-conjugated anti-CD31 labeling
- Method of euthanasia (CO2 overdose)
- Cell isolation procedure (positive immuno-selection with rat anti-mouse CD31)
- Cell culture conditions (EGM2 medium)
- Positive control: Wild-type littermates
- Negative control: Not explicitly mentioned
Annotations
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