Culturing Healthy Human iPSCs

The fully characterized hiPSC line is derived from dermal fibroblasts of a healthy male as previously published (Shinnawi et al., 2015 (link)). Informed consent was obtained after approval by the IRB committee of Amsterdam Medical Center. Colonies of hiPSCs were cultured in mTeSR-1 (StemCell Technologies) on plates coated with growth factor-reduced Matrigel (1:200 dilution, Corning). Cells were collected using 0.5 mM EDTA (Invitrogen), passaged every 4–6 days, and replated in mTeSR-1 supplemented with 2 μM thiazovivin (Selleck Chemicals). mTeSR-1 medium was replaced daily, except for the first day after passaging.

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Kremer V., Stanicek L., van Ingen E., Bink D.I., Hilderink S., Tijsen A.J., Wittig I., Mägdefessel L., Nossent A.Y, & Boon R.A. (2022). The long non-coding RNA MEG8 induces an endothelial barrier through regulation of microRNA-370 and -494 processing. Journal of Cell Science, 135(12), jcs259671.

Publication 2022

mTeSR-1 EDTA thiazovivin

Cells Dilution Edta Fibroblasts Growth factor Hipscs Male Matrigel Stemcell Thiazovivin

Corresponding Organization :

Other organizations : Vrije Universiteit Amsterdam, Amsterdam University Medical Centers, University of Amsterdam, Leiden University Medical Center, Goethe University Frankfurt, Klinikum rechts der Isar, Technical University of Munich, Karolinska Institutet, German Centre for Cardiovascular Research, Medical University of Vienna

Top 5 similar protocols

Variable analysis

independent variables

Passage of hiPSCs (every 4-6 days)
Supplementation of mTeSR-1 medium with 2 μM thiazovivin

dependent variables

Growth and proliferation of hiPSC colonies

control variables

Culture of hiPSCs in mTeSR-1 medium
Culture of hiPSCs on Matrigel-coated plates
Collection of hiPSCs using 0.5 mM EDTA
Replacement of mTeSR-1 medium daily, except for the first day after passaging

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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