Identifying K. pneumoniae from Clinical Samples

The clinical specimens were cultured on blood, chocolate, and MacConkey media except for urine processed on cystine lactose electrolyte deficient media (Nosheen et al., 2021 (link)). The identification of the bacteria was accomplished using Gram’s stain, morphology, oxidase, indole, citrate, sugar fermentation, and Microscan WalkAway Plus (Beckman Coulter, USA). A total of 79 K. pneumoniae strains obtained from inpatients and outpatients were isolated from the clinical specimens.

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, & Ejaz H. (2022). Analysis of diverse β-lactamases presenting high-level resistance in association with OmpK35 and OmpK36 porins in ESBL-producing Klebsiella pneumoniae. Saudi Journal of Biological Sciences, 29(5), 3440-3447.

Publication 2022

Microscan WalkAway Plus

Bacteria Chocolate Citrate Cultured blood Cystine Electrolyte Fermentation Gram stain Indole Inpatients K pneumoniae Lactose Outpatients Oxidase Strains Sugar Urine

Corresponding Organization : Al Jouf University

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Variable analysis

independent variables

Culturing clinical specimens on blood, chocolate, and MacConkey media
Processing urine specimens on cystine lactose electrolyte deficient media

dependent variables

Identification of bacteria using Gram's stain, morphology, oxidase, indole, citrate, sugar fermentation, and Microscan WalkAway Plus

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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