Creatinine-based eGFR Measurement in CKDGen Studies

The primary outcome of our GWAS meta-analysis is log-transformed eGFRcrea. This was used by the studies contributing to the CKDGen meta-analyses and for our UKB association analysis. In UKB, creatinine was measured in serum by enzymatic analysis on a Beckman Coulter AU5800 (UKB data field 30700, http://biobank.ctsu.ox.ac.uk/crystal/field.cgi?id=30700) and GFR was estimated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula^{53 (link),54 (link)}. For all studies involved in the CKDGen analysis, creatinine concentrations were measured in serum and GFR was estimated based on the CKD-EPI (for individuals >18 years of age)^{53 (link),54 (link)} or the Schwartz (for individuals <= 18 years of age)^{55 (link)} formula. Details on the study-specific measurements for the CKDGen studies were described previously^{7 (link)}. For all studies, eGFRcrea was winsorized at 15 or 200 ml/min/1.73 m² and winsorized eGFRcrea values were log-transformed using a natural logarithm. Secondary outcomes used for downstream analyses include log-transformed eGFRcys and log-transformed BUN. In UKB, cystatin C was measured based on latex enhanced immunoturbidimetric analysis on a Siemens ADVIA 1800 (UKB data field 30720, http://biobank.ctsu.ox.ac.uk/crystal/field.cgi?id=30720) and blood urea was measured by GLDH, kinetic analysis on a Beckman Coulter AU5800 (UKB data field 30670, http://biobank.ctsu.ox.ac.uk/crystal/field.cgi?id=30670). Details on the cystatin C and blood urea measurements in CKDGen studies can be found in the previous work^{7 (link),13 (link)}. In CKDGen and UKB, eGFRcys was obtained from cystatin C measurements using the formula by Stevens et al.^{56 (link)} or the CKD-EPI formula^{53 (link),54 (link)}, respectively. In all studies, eGFRcys was winsorized at 15 or 200 ml/min/1.73 m² and winsorized eGFRcys values were log-transformed using a natural logarithm. Blood urea measurements in mg/dL were multiplied by 2.8 to obtain BUN values, which were then log-transformed using a natural logarithm.

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Stanzick K.J., Li Y., Schlosser P., Gorski M., Wuttke M., Thomas L.F., Rasheed H., Rowan B.X., Graham S.E., Vanderweff B.R., Patil S.B., Robinson-Cohen C., Gaziano J.M., O’Donnell C.J., Willer C.J., Hallan S., Åsvold B.O., Gessner A., Hung A.M., Pattaro C., Köttgen A., Stark K.J., Heid I.M, & Winkler T.W. (2021). Discovery and prioritization of variants and genes for kidney function in >1.2 million individuals. Nature Communications, 12, 4350.

Publication 2021

Blood Creatinine Cystatin c Enzymatic Gwas Immunoturbidimetric Kinetic Latex Serum Urea Urea in blood

Corresponding Organization :

Other organizations : University of Regensburg, University Medical Center Freiburg, University of Freiburg, Norwegian University of Science and Technology, Vanderbilt University Medical Center, University of Michigan–Ann Arbor, VA Tennessee Valley Healthcare System, Vanderbilt University, VA Boston Healthcare System, University Hospital Regensburg, Institute for Biomedicine, Eurac Research

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Log-transformed eGFRcrea
Log-transformed eGFRcys
Log-transformed BUN

control variables

Creatinine measured in serum by enzymatic analysis on a Beckman Coulter AU5800 in UKB
GFR estimated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula in UKB and CKDGen studies
Cystatin C measured based on latex enhanced immunoturbidimetric analysis on a Siemens ADVIA 1800 in UKB
Blood urea measured by GLDH, kinetic analysis on a Beckman Coulter AU5800 in UKB
EGFRcys obtained from cystatin C measurements using the formula by Stevens et al. in CKDGen or the CKD-EPI formula in UKB
EGFRcrea and eGFRcys winsorized at 15 or 200 ml/min/1.73 m^2 and log-transformed using natural logarithm
BUN values obtained by multiplying blood urea measurements in mg/dL by 2.8 and then log-transformed using natural logarithm

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