MAPK15 Expression Vectors for Cisplatin Sensitivity

The pCEFL HA, pCEFL HA MAPK15 [18 (link)], pCEFL HA MAPK15_AXXA and pCEFL HA MAPK15_KD [25 (link)] expression vectors have been previously described. The corresponding cDNA (MAPK15_WT, MAPK15_AXXA and MAPK15_KD), resistant to siRNA for MAPK15 (siMAPK15-1), were synthesized (GeneART, Thermo Fischer Scientific) with “synonymous” mutations in the sequence recognized by the siRNA and cloned in the pCEFL HA expression vector. The identity and integrity of all vectors was confirmed by DNA sequencing. Cis-platin (Cis-Pt; Sigma Aldrich, Milan, Italy) was used at a final concentration of 1 μM. Bafilomycin A₁ (Santa Cruz Biotechnology, Heidelberg, Germany) and RO31-8220 (Merck Millipore, Vimodrone, Italy) were used at a final concentration of 100 nM.

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Rossi M., Colecchia D., Ilardi G., Acunzo M., Nigita G., Sasdelli F., Celetti A., Strambi A., Staibano S., Croce C.M, & Chiariello M. (2016). MAPK15 upregulation promotes cell proliferation and prevents DNA damage in male germ cell tumors. Oncotarget, 7(15), 20981-20998.

Publication 2016

GeneART Cis-platin (Cis-Pt; Bafilomycin A1 RO31-8220

Bafilomycin a1 Cdna Cis platin Mapk15 Ro31 8220 Sirna Synonymous mutations Vectors

Corresponding Organization : Istituto di Fisiologia Clinica

Other organizations : The Ohio State University, Institute for Experimental Endocrinology and Oncology

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Variable analysis

independent variables

PCEFL HA
PCEFL HA MAPK15 [18]
PCEFL HA MAPK15_AXXA
PCEFL HA MAPK15_KD [25]
MAPK15_WT
MAPK15_AXXA
MAPK15_KD

dependent variables

Not explicitly mentioned

control variables

Cis-platin (Cis-Pt) at 1 μM
Bafilomycin A1 at 100 nM
RO31-8220 at 100 nM

controls

Positive control: Not specified
Negative control: Not specified

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