Cytokine and Nitrite Quantification

The cytokines TNF-α, IL-6, and IL-10 were determined using BD CBA Mouse Cytokine Assay kits according to the manufacturer's specification (BD Biosciences, CA, USA). Flow cytometry measurements were performed on a FACSCalibur flow cytometry (BD Bioscience, CA, USA) collected using the Cell-QuestTM software package provide by the manufacturer (1200 events). FlowJO software 7.6.4 (Tree Star, Inc, Ashland, OR) was used for data analysis. Results are representative of two experiments in triplicate. Nitrite concentration (NO) was determinate by Griess Reaction as described (Nogueira et al., 2015 (link)).

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Nogueira P.M., de Menezes-Neto A., Borges V.M., Descoteaux A., Torrecilhas A.C., Xander P., Revach O.Y., Regev-Rudzki N, & Soares R.P. (2020). Immunomodulatory Properties of Leishmania Extracellular Vesicles During Host-Parasite Interaction: Differential Activation of TLRs and NF-κB Translocation by Dermotropic and Viscerotropic Species. Frontiers in Cellular and Infection Microbiology, 10, 380.

Publication 2020

BD CBA Mouse Cytokine Assay kits FACSCalibur flow cytometry

Assay Cba mouse Cell Cytokine Flow cytometry Il 10 Nitrite Tnf α Tree

Corresponding Organization : Fundação Oswaldo Cruz

Other organizations : Institut National de la Recherche Scientifique, Instituto de Ciências Farmacêuticas, Universidade Federal de São Paulo, Weizmann Institute of Science

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Variable analysis

independent variables

TNF-α
IL-10

dependent variables

Cytokine levels
Nitrite concentration (NO)

control variables

Flow cytometry measurements performed on a FACSCalibur flow cytometry (BD Bioscience, CA, USA)
Cell-QuestTM software package provided by the manufacturer (1200 events)
FlowJO software 7.6.4 (Tree Star, Inc, Ashland, OR) used for data analysis

controls

Positive control: Not specified
Negative control: Not specified

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