The headspace compounds from cell
culture vials were analyzed using SPME-GC/MS as described before.6 (link) In brief, a 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane
fiber (length 1 cm; Supelco, Steinheim, Germany) was used to absorb
the terpene compounds at 60 °C for 20 min. Following that, 1
min desorption of compounds in the split inlet (250 °C; SPME
liner, 0.75 mm i.d.; Supelco) and analysis by an Agilent 7980B GC
equipped with an Agilent 5977B MSD was done. An Agilent DB5ms column
was injected with samples (split ratio of 40:1 at 240 °C). The
oven heating program started at 80 °C for 1 min followed by a
ramp to 210 °C at 10 °C/min, then to 310 °C at 60 °C/min,
and finally held at 310 °C for 2 min. The peaks were detected
using a mass spectrometer operated in EI mode with full scan analysis
(m/z 33–300, 9 scans/s) and
identified using the National Institute of Standards and Technology
(NIST) database (for β-elemene, α-selinene, and β)
or by authentic chemical standards (for linalool and nerolidol6 (link)).
culture vials were analyzed using SPME-GC/MS as described before.6 (link) In brief, a 50/30 μm divinylbenzene/carboxen/polydimethylsiloxane
fiber (length 1 cm; Supelco, Steinheim, Germany) was used to absorb
the terpene compounds at 60 °C for 20 min. Following that, 1
min desorption of compounds in the split inlet (250 °C; SPME
liner, 0.75 mm i.d.; Supelco) and analysis by an Agilent 7980B GC
equipped with an Agilent 5977B MSD was done. An Agilent DB5ms column
was injected with samples (split ratio of 40:1 at 240 °C). The
oven heating program started at 80 °C for 1 min followed by a
ramp to 210 °C at 10 °C/min, then to 310 °C at 60 °C/min,
and finally held at 310 °C for 2 min. The peaks were detected
using a mass spectrometer operated in EI mode with full scan analysis
(m/z 33–300, 9 scans/s) and
identified using the National Institute of Standards and Technology
(NIST) database (for β-elemene, α-selinene, and β)
or by authentic chemical standards (for linalool and nerolidol6 (link)).
