Mouse Ovary Isolation and Culture

The ovary isolation and culture were performed as reported by Cai et al. [49 (link)]. Briefly, the ovaries from 12.5, 13.5, 14.5, or 15.5 dpc mice were separated and washed by prechilled PBS three times, which were then cultured in 6-well culture dishes containing 1 ml of basic DMEM/F12 medium (Gibco, Life Technologies) at 37 °C in 5% CO₂/95% air atmosphere with saturated humidity. Half of the medium was replaced every 2 days until the ovaries grew to the required stages. 25 μM D4476 was added to detect the effects of CK1α on folliculogenesis.

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Zhang D., Jiang Y., Luo X., Liu H., Zhou Y, & Cui S. (2022). Oocyte Casein kinase 1α deletion causes defects in primordial follicle formation and oocyte loss by impairing oocyte meiosis and enhancing autophagy in developing mouse ovary. Cell Death Discovery, 8, 388.

Publication 2022

basic DMEM/F12 medium

Atmosphere Ck1α Dishes Humidity Isolation Mice Ovaries

Corresponding Organization :

Other organizations : Yangzhou University, China Agricultural University

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Variable analysis

independent variables

Developmental stage of mouse ovaries (12.5, 13.5, 14.5, or 15.5 dpc)
Addition of 25 μM D4476 to detect the effects of CK1α on folliculogenesis

dependent variables

Growth and development of mouse ovaries

control variables

Basic DMEM/F12 medium
Culture conditions (37 °C, 5% CO2/95% air atmosphere with saturated humidity)
Half-medium replacement every 2 days

controls

Negative control: Ovaries cultured without the addition of 25 μM D4476

Annotations

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Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

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