INS-1 832/13 β-cells were trypsinized from the growth plate and stained per the manufacturer’s recommendation using a CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit to test Caspase-3/7 activity (Invitrogen, Cat. No. C10427) [26 (link)]. After staining, data were acquired using an Attune NxT cytometer (Invitrogen). Data were analyzed using FlowJo (BD Life Sciences, Franklin Lakes, NJ, USA, version 10.8.1).
Free full text: Click here