Cytokine and Chemokine Profiling in Colonic Tissues

Colonic tissues were lysed in Cell Lytic Mammalian Tissue Lysis Extraction Reagent (Sigma) and analyzed using the 25-analyte MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel (EMD Millipore) on a FLEXMAP 3D instrument (Luminex) (21 (link), 22 (link)). Data were standardized to tissue protein concentrations measured by the BCA Protein Assay Kit (Pierce). TNF-α and IFN-γ in tissues was measured using R&D DuoSet ELISA kits (10 (link)).

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Singh K., Coburn L.A., Asim M., Barry D.P., Allaman M.M., Shi C., Washington M.K., Luis P.B., Schneider C., Delgado A.G., Piazuelo M.B., Cleveland J.L., Gobert A.P, & Wilson K.T. (2018). Ornithine Decarboxylase in Macrophages Exacerbates Colitis and Promotes Colitis-Associated Colon Carcinogenesis by Impairing M1 Immune Responses. Cancer research, 78(15), 4303-4315.

Publication 2018

Cell Lytic Mammalian Tissue Lysis Extraction Reagent MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel

Assay Cell Chemokine Colonic Cytokine Elisa Ifn γ Mammalian Mouse Protein Tissues Tnf α

Corresponding Organization : VA Tennessee Valley Healthcare System

Other organizations : Vanderbilt University, Moffitt Cancer Center

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Cytokine/chemokine levels in colonic tissues
TNF-α and IFN-γ levels in tissues

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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