Drug-Induced Transcriptional Analysis of C. albicans

To investigate the mRNA expression of SPE1, DUR3, and FLU1 upon drug treatment, real-time RT-PCR experiment was conducted on a 7500 real-time PCR system (Applied Biosystems) as described previously [19 (link)]. C. albicans SC5314 cells were treated with 8 μg/ml MG, 0.125 μg/ml CAS alone or in combination for 4 h. The primers for SPE1, DUR3, and FLU1 are listed in Table S1 in the supplemental material. The fungal RNAout kit (TIANZ, Beijing, China) was used for total RNA isolation. The cDNA was acquired with the cDNA Synthesis Kit for RT-PCR (TaKaRa Biotechnology, Dalian, China). The amplified products in real time were monitored with SYBR green I (TaKaRa Biotechnology, Dalian, China). The expression of the genes was normalized to that of 18S rRNA.