FGFR1 Inhibition Effects on Oligodendrocytes

PD166866, a potent FGFR1 tyrosine kinase inhibitor, was used in this study. PD166866 was purchased from Sigma-Aldrich (Steinheim, Germany) and dissolved in DMSO, and above 80% confluent cells were treated with a 10 µM concentration of PD166866 for 3 h. Effects of IFNβ-1a and FGFR1 inhibition on proliferation, cytotoxicity, FGFR signaling, and TrkB/BDNF protein expression in Oli-neu oligodendrocytes were analyzed. Oli-neu culture was performed as described previously [41 (link)]. Briefly, experiments were performed at passages 8–12. To assess the effects of IFNβ-1a and FGFR1 inhibition on cell proliferation, FGFR signaling, and myelin proteins, Oli-neu cells were treated with FGF2 (20 ng/mL), PD166866 (10 μM), IFNβ-1a (400 ng/mL, Rebif^®), IFNβ-1a + PD166866, and DMSO. For FGFR signaling activation, stimulation with FGF2 was performed. After 24 h of incubation, protein isolation and western blot were carried out [41 (link)].