Whole tissue supernatants were prepared as described previously [40 (link)]. Testicular levels of interleukin-6 (IL-6) (BMS625 IL-6 rat Elisa kit, Thermo Fisher Scientific, Waltham, MA, USA) and TNF-α (BMS 622 rat TNF-α kit, Thermo Fisher Scientific, Waltham, MA, USA) were measured by enzymatic method using commercial kits as per the manufacturer’s instructions. IL-6 and TNF-α concentrations are expressed per milligram protein.
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