Methanol, methyl tert-butyl ether, acetonitrile, water, hexane, acetone, formic acid and ammonium were of HPLC grade and purchased from Fisher Scientific. Beta-carotene was purchased from Sigma Aldrich, soy isoflavones (genistein, genistin, daidzein, daidzin, glycitein, and glycitin) were from ChromaDex, and lycopene was isolated and purified from tomato52 (link).
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