Western Blotting for Liver Protein Analysis

Western blotting for liver proteins was performed as reported previously (25 (link)). Primary antibody for apoB was from L. Swift of Vanderbilt University School of Medicine (26 (link)). Primary antibody for CETP was from Abcam (ab-51771), rabbit anti-mouse apoA1 was from Meridian Life Science (K23500R), antibody for apoM was from Cell Signaling (5709S), goat anti-mSAA1 was from R&D Systems (AF2948), antibody for LDL receptor was from Abcam (ab30532), rabbit polyclonal anti-PLTP antibody was from Abcam (ab189776), and rabbit anti-actin (I-19) antibody was from Santa Cruz (sc-1616). Secondary antibodies were from LI-COR (Lincoln, NE). All primary antibodies were diluted 1:1,000, except where noted. All secondary antibodies were diluted 1:10,000. Images were acquired using a LI-COR Odyssey infrared imaging.

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Zhu L., Luu T., Emfinger C.H., Parks B.A., Shi J., Trefts E., Zeng F., Kuklenyik Z., Harris R.C., Wasserman D.H., Fazio S, & Stafford J.M. (2018). CETP Inhibition Improves HDL Function but Leads to Fatty Liver and Insulin Resistance in CETP-Expressing Transgenic Mice on a High-Fat Diet. Diabetes, 67(12), 2494-2506.

Publication 2018

AF2948 ab30532 ab189776 sc-1616 Odyssey infrared imaging

Actin Anti antibody Antibodies Antibody Apoa1 Apob Apom Goat Liver Meridian Mouse Proteins Rabbit Receptor antibody

Corresponding Organization : Vanderbilt University

Other organizations : Centers for Disease Control and Prevention, Trinity College, Duke University, Oregon Health & Science University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Primary antibody for apoB from L. Swift of Vanderbilt University School of Medicine
Primary antibody for CETP from Abcam (ab-51771)
Rabbit anti-mouse apoA1 from Meridian Life Science (K23500R)
Antibody for apoM from Cell Signaling (5709S)
Goat anti-mSAA1 from R&D Systems (AF2948)
Antibody for LDL receptor from Abcam (ab30532)
Rabbit polyclonal anti-PLTP antibody from Abcam (ab189776)
Rabbit anti-actin (I-19) antibody from Santa Cruz (sc-1616)

dependent variables

Liver protein expression levels

control variables

Western blotting protocol as reported previously (25)
Secondary antibodies from LI-COR (Lincoln, NE)
All primary antibodies diluted 1:1,000, except where noted
All secondary antibodies diluted 1:10,000
Images acquired using a LI-COR Odyssey infrared imaging

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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