iPSCs were generated from dermal fibroblasts from two patients with wet AMD associated with the homozygous CFH Y402H polymorphism (high‐risk AMD RPE) and two age matched donors with no clinical or genotypic indication of ocular disease (low‐risk control RPE), as reported previously (Hallam et al., 2017 (link)). Two additional control cell lines (WT1 and WT3) were used to confirm the reproducibility of our iPSC and iPSC‐RPE culture (Buskin et al., 2018 ). iPSCs were cultured in mTeSR1 (Stem Cell Technologies) growth media on Matrigel Growth Factor Reduced Basement Membrane Matrix (Corning) in humidified, 5% CO2, 37°C conditions. iPSCs were passaged every 4–6 days using Versene (EDTA) 0.02% (Lonza).
Kurzawa‐Akanbi M., Whitfield P., Burté F., Bertelli P.M., Pathak V., Doherty M., Hilgen B., Gliaudelytė L., Platt M., Queen R., Coxhead J., Porter A., Öberg M., Fabrikova D., Davey T., Beh C.S., Georgiou M., Collin J., Boczonadi V., Härtlova A., Taggart M., Al‐Aama J., Korolchuk V.I., Morris C.M., Guduric‐Fuchs J., Steel D.H., Medina R.J., Armstrong L, & Lako M. (2022). Retinal pigment epithelium extracellular vesicles are potent inducers of age‐related macular degeneration disease phenotype in the outer retina. Journal of Extracellular Vesicles, 11(12), 12295.
Other organizations :
University of Glasgow, Queen's University Belfast, University of the Highlands and Islands, Loughborough University, University of Gothenburg, University Medical Center Freiburg, Institute of Medical Microbiology and Hygiene, King Abdulaziz University
Passaging of iPSCs (every 4-6 days using Versene (EDTA) 0.02%)
positive controls
Two additional control cell lines (WT1 and WT3) used to confirm the reproducibility of iPSC and iPSC-RPE culture
negative controls
No negative controls explicitly mentioned
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