Necropsy was performed on all subjects. Tissue samples of all major organs were collected for histopathologic and immunohistochemical examination, immersion-fixed in 10% neutral buffered formalin, and processed for histopathology as previously described16 (link),17 (link). For immunohistochemistry, specific anti-EBOV immunoreactivity was detected using an anti-EBOV VP40 protein rabbit primary antibody (Integrated BioTherapeutics) at a 1:4000 dilution. In brief, tissue sections were processed for immunohistochemistry using the Dako Autostainer (Dako). Secondary antibody used was biotinylated goat anti-rabbit IgG (Vector Laboratories) at 1:200 followed by Dako LSAB2 streptavidin-HRP (Dako). Slides were developed with Dako DAB chromagen (Dako) and counterstained with hematoxylin. Non-immune rabbit IgG was used as a negative control. Liver, adrenal gland, and inguinal lymph nodes representative images were taken at 40×, and spleen taken at 20× from control animal 0805068 (A, E, H, and M) or treated animals 0902056 (B, F, J, and N), 1005445 (C, G, K, and O), and 1006421 (D, H, L, and P).