Primary Mouse Skeletal Muscle Cell Isolation

Primary mouse skeletal muscle cells (from Barx +/− animals, and between 14 and 16 passage doubling) were isolated as previously described (Rando and Blau, 1994 ; Meech et al., 2012 (link)) and cultured on MatTek dishes treated as described above for C2C12 cells. Myoblasts were cultured in Growth Medium (40% Ham’s F-10 (Sigma-Aldrich) and DMEM supplemented with 20% FBS, 5% Fungizone (Invitrogen), 1% penicillin/streptomycin, and 2.5 ng FGF (Fibroblast Growth Factor, Basic Human (Cat #: G507A, Promega; Madison, WI)) in 5% CO₂ at 37°C. The Growth Medium was removed after 2–3 days and the same Differentiation Medium used for the C2C12 cells was added to the cultures to promote myotube formation.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

White J., Barro M.V., Makarenkova H.P., Sanger J.W, & Sanger J.M. (2014). Localization of sarcomeric proteins during myofibril assembly in cultured mouse primary skeletal myotubes. Anatomical record (Hoboken, N.J. : 2007), 297(9), 1571-1584.

Publication 2014

Ham’s F-10 Fungizone Fibroblast Growth Factor,

Animals Cells Dishes Fibroblast growth factor Fungizone Human Mouse Myoblasts Myotube Penicillin Promega Streptomycin

Corresponding Organization : SUNY Upstate Medical University

Other organizations : Scripps Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Genotype of the primary mouse skeletal muscle cells (Barx +/− animals)

dependent variables

Myotube formation

control variables

Passage doubling of the primary mouse skeletal muscle cells (between 14 and 16 passages)
Culture conditions (Growth Medium and Differentiation Medium)
Cell type (primary mouse skeletal muscle cells)

controls

Positive control: C2C12 cells, which were treated and cultured under the same conditions as the primary mouse skeletal muscle cells
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!