Viral nucleic acid was extracted from sentinel samples using the MagNA PURE 96 system (Roche, Mannheim, Germany). Real-time RT-PCR reactions were conducted using primers targeting the SARS-CoV-2 envelope (E) and nucleoprotein (N) genes based on a previously described protocol [8 (link)]. All RT-PCR reactions were conducted using the Ambion Ag-Path Master Mix (Life Technologies, Austin, Texas, United States (US)) and TaqMan Chemistry on ABI 7500 instruments.
Glatman-Freedman A., Gur-Arie L., Sefty H., Kaufman Z., Bromberg M., Dichtiar R., Rosenberg A., Pando R., Nemet I., Kliker2, L., Mendelson E., Keinan-Boker L., Zuckerman N.S, & Mandelboim M. (2022). The impact of SARS-CoV-2 on respiratory syndromic and sentinel surveillance in Israel, 2020: a new perspective on established systems. Eurosurveillance, 27(16), 2100457.
Viral nucleic acid extraction using the MagNA PURE 96 system (Roche, Mannheim, Germany)
dependent variables
SARS-CoV-2 envelope (E) and nucleoprotein (N) gene expression detected by real-time RT-PCR
control variables
Use of primers targeting the SARS-CoV-2 envelope (E) and nucleoprotein (N) genes based on a previously described protocol [8]
Use of Ambion Ag-Path Master Mix (Life Technologies, Austin, Texas, United States (US)) and TaqMan Chemistry on ABI 7500 instruments for RT-PCR reactions
controls
No positive or negative controls were explicitly mentioned in the provided information.
Annotations
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