Isolation of fucoxanthin from C. striata microalgae was carried out through several steps, including cultivation, extraction, and purification. Initially, the stock solution of C. striata was cultivated in seawater media for 14 days referring to the modified method of Kusumaningtyas et al. (2017) . On the 14 th day after cultivation, the seawater culture was centrifuged to obtain C. striata microalgae biomass. A total of 6.1 g of biomass was extracted by 18 mL of ethanol and then centrifuged to separate extract and waste (Perez et al., 2019) . The fucoxanthin extract was purified using medium-pressure liquid chromatography (MPLC). Approximately 1 mL of ethanol extract was injected into the MPLC at a flow rate of 25 mL/min. The sample was then fractionated using SiO 2 as the stationary phase and ethanol as the mobile phase. The detector used was a photodiode array at wavelengths 220, 254, and 364 nm. The MPLC fractions of fucoxanthin were then characterized using a UV-Vis spectrophotometer and stored in a dark bottle.