Maintenance and Characterization of iPSCs

Induced pluripotent stem cells (iPSCs) were maintained in Essential 8 (Life Technologies, Carlsbad, CA, USA) on Matrigel (Corning, Corning, NY, USA) coated 6-well plates (VWR, Radnor, PA, USA) and passaged every 4–6 days with Versene (Life Technologies, Carlsbad, CA, USA). Cultures were routinely tested for mycoplasm using the Mycoalert Detection Kit (Lonza, Basel, Switzerland). Two independent control lines [16 (link),20 (link)], one N139K SOD1 line [21 (link)], one A4V SOD1 line (Coriell, Camden, NJ, USA), and two independent C9orf72 lines [22 (link)] were also used.

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Seminary E.R., Santarriaga S., Wheeler L., Mejaki M., Abrudan J., Demos W., Zimmermann M.T., Urrutia R.A., Fee D., Barkhaus P.E, & Ebert A.D. (2020). Motor Neuron Generation from iPSCs from Identical Twins Discordant for Amyotrophic Lateral Sclerosis. Cells, 9(3), 571.

Publication 2020

Essential 8 Matrigel 6-well plates Versene Mycoalert Detection Kit

Induced pluripotent stem cells Matrigel Mycoplasm Versene

Corresponding Organization : Medical College of Wisconsin

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Variable analysis

independent variables

Cell line type (control lines, N139K SOD1 line, A4V SOD1 line, C9orf72 lines)

dependent variables

Not explicitly mentioned

control variables

Culture conditions (Essential 8 medium, Matrigel-coated 6-well plates, passaged every 4-6 days with Versene)
Mycoplasma testing

controls

Positive controls: Two independent control lines (16, 20)
Negative controls: Not explicitly mentioned

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