Cardiac Function Assessment in Rats

Rats were anaesthetized with a mixture of 800 mg/kg urethane (SIGMA, USA) and 40 mg/kg α-chloralose (SIGMA, USA). A transducer catheter (SPR-869, MILLAR INSTRUMENTS) was introduced into the left ventricle through the right carotid. Cardiac left ventricle pressure-volume (PV) loops were recorded at steady state. Measurements were calibrated by injecting a hypertonic saline bolus (30% wt/vol NaCl) to determine conductance, and relative volume units were converted absolute using the cuvette calibration method. The LV end-of-systole volume (LVESV), LV end-diastolic volume (LVEDV), LV end-systole pressure (LVESP), LV end-diastolic pressure (LVEDP), ejection volume (SV), cardiac output (CO) and ejection fraction (EF) were obtained from 10–15 representative loops. Volume at pressure 0 and End-Systolic Pressure-Volume Relationship (ESPVR) were used as indicators of diastolic and systolic function, respectively. These analyses were calculated by a mathematical algorithm from a single beat obtained from pressure-volume curves according to Klotz et al.^{21 (link)} for V-0 and Takeushi et al.^{22 (link)} for ESPVR. All recordings were sampled to 1 kHz and were analyzed using LABCHART7 Pro v7.2 software (ADINSTRUMENTS)^{18 (link),19 (link),21 (link),22 (link),49 (link)}.

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Lucero C.M., Andrade D.C., Toledo C., Díaz H.S., Pereyra K.V., Diaz-Jara E., Schwarz K.G., Marcus N.J., Retamal M.A., Quintanilla R.A, & Del Rio R. (2020). Cardiac remodeling and arrhythmogenesis are ameliorated by administration of Cx43 mimetic peptide Gap27 in heart failure rats. Scientific Reports, 10, 6878.

Publication 2020

urethane α-chloralose SPR-869

Cardiac output Cardiac volume Carotid Catheter Chloralose Diastolic Diastolic pressure Hypertonic saline Left ventricle Nacl Pressure Rats Systolic Systolic pressure Transducer Urethane

Corresponding Organization : Pontificia Universidad Católica de Chile

Other organizations : Des Moines University, Clínica Alemana, Universidad del Desarrollo, Universidad Autónoma de Chile

Top 5 similar protocols

Variable analysis

independent variables

Urethane (800 mg/kg)
α-chloralose (40 mg/kg)

dependent variables

Left ventricular end-of-systole volume (LVESV)
Left ventricular end-diastolic volume (LVEDV)
Left ventricular end-systole pressure (LVESP)
Left ventricular end-diastolic pressure (LVEDP)
Ejection volume (SV)
Cardiac output (CO)
Ejection fraction (EF)
Volume at pressure 0
End-Systolic Pressure-Volume Relationship (ESPVR)

control variables

Anaesthesia protocol (urethane and α-chloralose)
Transducer catheter (SPR-869, MILLAR INSTRUMENTS)
Injection of hypertonic saline bolus (30% wt/vol NaCl) for calibration
Cuvette calibration method
Pressure-volume (PV) loop recording at steady state
10-15 representative loops analyzed
Sampling rate of 1 kHz
LABCHART7 Pro v7.2 software for analysis

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