Mouse pups were initially anesthetized with isoflurane, fixed into the stereotaxic apparatus, and local anesthetic was administered. Multi-site electrodes (NeuroNexus, Ann Arbor, MI) were inserted to the skull surface into the prefrontal cortex (PFC) and hippocampus (HC). Two silver wires were inserted into cerebellum and served as ground and reference electrodes. Simultaneous recordings of local field potential (LFP) and multi-unit activity were performed from the prelimbic subdivision of the PFC and the CA1 area of the intermediate HC as described [58 (link)]. See supplementary methods for further detail.
Other organizations :
University Medical Center Hamburg-Eppendorf, Universität Hamburg, McMaster University, Hospital for Sick Children, SickKids Foundation, University of Toronto
Insertion of multi-site electrodes into the prefrontal cortex (PFC) and hippocampus (HC)
dependent variables
Local field potential (LFP)
Multi-unit activity
control variables
Anesthetization of mouse pups with isoflurane
Fixation of mouse pups into the stereotaxic apparatus
Administration of local anesthetic
Insertion of two silver wires into the cerebellum to serve as ground and reference electrodes
controls
Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
