Respiration was measured in freely moving mice using plethysmography chambers (EMKA Technologies, Paris, France) supplied with room air as described previously (Hill et al., 2016 (link), 2018 (link)). Mice were habituated to respiratory chambers for 30 min, on the day prior to experimentation. Respiratory parameters were recorded (IOX software ‐ EMKA Technologies, Paris, France) and averaged over 5 min periods.
Data are presented both as minute volume (MV) and as percentage change from the pre‐drug MV baseline, calculated from data for each individual mouse before collating and plotting as a mean. Mice within cohorts may vary in size and this varies their individual MVs, accordingly; data are presented as a percentage change from each mouse's pre‐drug baseline controls for these inherent variations.
Data are presented both as minute volume (MV) and as percentage change from the pre‐drug MV baseline, calculated from data for each individual mouse before collating and plotting as a mean. Mice within cohorts may vary in size and this varies their individual MVs, accordingly; data are presented as a percentage change from each mouse's pre‐drug baseline controls for these inherent variations.
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