To obtain the chemical composition of the cyanobacterium, the amounts of total nitrogen (N), nitrite (NO2−), nitrate (NO3−), ammonium (NH4+), sulfate (SO42−), phosphate (PO43−), carbonate (CO32−), magnesium (Mg2+), calcium (Ca2+), sodium (Na+), and potassium (K+) ions were determined at the Institute of Arian Fan Azma, Iran, following the methods summarized in Table 2 . Each test was carried out in three replications, and the averages of the obtained amounts were provided.
Chemical content of cyanobacteria
| Analytical Method | Nostoc spongiaeforme ISB65 | |
|---|---|---|
| Total N (mg.L− 1) | Macro kjeldahl | 35.00 |
| NO2− (mg.L− 1) | Colorimetric | 0.60 |
| NO3− (mg.L− 1) | Ultraviolet Spectrophotometric | 9.40 |
| NH4+ (mg.L− 1) | Nesslerization | 5.00 |
| Phosphate (mg.L− 1) | Vanadomolybdophosphoric acid colorimetric | 9.00 |
| CO32− (mg.L− 1) | Titrimetric | 0.00 |
| HCO3− (mg.L− 1) | Titrimetric | 30.00 |
| Na+ (mg.L− 1) | Flame Emission Photometric | 1.50 |
| Mg2+ (mg.L− 1) | EDTA Titrimetric | 5.00 |
| Ca2+ (mg.L− 1) | EDTA Titrimetric | 7.00 |
| K+ (mg.L− 1) | Flame Emission Photometric | 0.11 |
| EC (µS cm− 1) | Platinum Electrode | 15.00 |
| pH | Electrometric | 6.02 |
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