Using a scalpel, a longitudinal piece of each organ was harvested and weighed. Samples weighed between 0.01 g and 0.26 g. After weighing, the samples were added to a centrifuge tube. 500 μl of Aqua Regia (1 : 3 nitric acid : hydrochloric acid) was added to begin digestion. A glass rod was used to break down the sample into small pieces. The samples were allowed to digest for 1–7 days. Samples were diluted to 5% Aqua Regia with purified DI water. Samples were mixed by vortexing and then filtered through a 1.0 μm Nylon syringe filter before testing. The samples were run on a Perkins Elmer Avio 200 using a 5-point standard curve created from Si, Ag, and Au standards (LabChem). The instrument was blanked using a 5% Aqua Regia and purified DI water solution. The concentrations of elements in each sample were related to the weight of digested samples.
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Elemental Analysis of Organ Samples
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Corresponding Organization :
Other organizations : University of Notre Dame
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Variable analysis
independent variables
- Organ type (not explicitly stated, but can be inferred from the description of harvesting a longitudinal piece of each organ)
- Weights of the organ samples (ranging from 0.01 g to 0.26 g)
- Digestion method (using Aqua Regia, a specific acid mixture of nitric acid and hydrochloric acid)
- Digestion duration (1-7 days)
- Dilution of the samples (to 5% Aqua Regia)
- Filtration method (using a 1.0 μm Nylon syringe filter)
- Analytical method (Perkins Elmer Avio 200 using a 5-point standard curve created from Si, Ag, and Au standards)
- Blanking solution (5% Aqua Regia and purified DI water)
- Positive controls: Not explicitly mentioned
- Negative controls: Not explicitly mentioned
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