Amyloid-beta Induced Neuronal Damage

HT-22 hippocampal cells (HT-22 cells) were obtained from the Chinese Academy of Sciences (Shanghai, China), and the cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM) with 10% Fetal Bovine Serum (FBS), 1% penicillin and 1% streptomycin in a humidified 5% CO₂ atmosphere at 37°C. Cells were treated with 5 μM, 10 μM, and 20 μM Aβ1-42 oligomer, respectively. Aβ1-42 oligomer preparation method: Aβ1-42 (Abcam, USA) dissolved in DMSO, ultrasonic 5 min in the cold bath, then immediately stored at -80°C. The Aβ1-42/DMSO solution was diluted with serum-free DMEM to a final concentration of 100 μM and stored at 37°C.¹⁴ (link)