Cell-laden hydrogels were either cultured in pro-inflammatory
media, which consisted of the cell expansion medium supplemented with
50 μg/mL ascorbic acid (Millipore Sigma) and 20 ng/mL recombinant
human IL-1β (Peprotech) to simulate the osteoarthritic environment,49 (link) or in noninflammatory media, which consisted
of the cell expansion medium supplemented with 50 μg/mL ascorbic
acid and 40 ng/mL dexamethasone (Millipore Sigma).50 (link) Hydrogels were cultured in 48-well plates with media exchanged
and collected every 2 days. fbACs were encapsulated at a final cell
concentration of 2 million cells/mL.
media, which consisted of the cell expansion medium supplemented with
50 μg/mL ascorbic acid (Millipore Sigma) and 20 ng/mL recombinant
human IL-1β (Peprotech) to simulate the osteoarthritic environment,49 (link) or in noninflammatory media, which consisted
of the cell expansion medium supplemented with 50 μg/mL ascorbic
acid and 40 ng/mL dexamethasone (Millipore Sigma).50 (link) Hydrogels were cultured in 48-well plates with media exchanged
and collected every 2 days. fbACs were encapsulated at a final cell
concentration of 2 million cells/mL.
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