Protocol detail

Cellular Subset Depletion Protocol

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For cellular subsets depletion experiments, mice were injected intraperitoneally with 400 μg depletion antibody twice weekly beginning 1 day before therapy. The depletion antibody are as follows: anti-CD4 (clone GK1.5, BioXCell), anti-CD8α (clone 2.43, BioXCell), anti-NK1.1 (clone PK136, BioXCell) antibodies and isotype control antibody (clone LTF-2, BioXCell) 16 (link). The depletion efficacy was confirmed by flow cytometry of PBMC on day 14 (Figure S9).

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Yu X., Yu J., Dai H., Deng C., Sun X., Long S., Jiang Z., Jin H., Guan Z, & Yang Z. (2022). Novel formulation of c-di-GMP with cytidinyl/cationic lipid reverses T cell exhaustion and activates stronger anti-tumor immunity. Theranostics, 12(15), 6723-6739.

Publication 2022

anti-CD4 (clone GK1.5, anti-CD8α (clone 2.43, anti-NK1.1 (clone PK136, isotype control antibody (clone LTF-2,

Antibodies Antibody Cellular Clone Flow cytometry Isotype Mice Therapy

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Variable analysis

independent variables

Anti-CD4 (clone GK1.5, BioXCell) antibody
Anti-CD8α (clone 2.43, BioXCell) antibody
Anti-NK1.1 (clone PK136, BioXCell) antibody
Isotype control antibody (clone LTF-2, BioXCell)

dependent variables

Depletion efficacy

control variables

Mice were injected intraperitoneally with 400 μg depletion antibody twice weekly beginning 1 day before therapy

controls

Isotype control antibody (clone LTF-2, BioXCell)

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