Thermal hyperalgesia was tested with a Hargreaves heat source by using the Plantar Test apparatus (Ugo Basile, Gemonio, Italy) [13 (link),14 (link)]. Each paw was tested 5 times with at least 2 min break in between. The lowest and highest reflex latency scores of each paw were discarded and the bilateral mean was calculated and plotted. The behavioral test was performed before SCI and once a week on each animal when mice reached a BMS score of 5.
Time of testing (before SCI and once a week after reaching BMS score of 5)
dependent variables
Bilateral mean of reflex latency scores
control variables
Plantar Test apparatus (Ugo Basile, Gemonio, Italy)
Thermal hyperalgesia testing using Hargreaves heat source
Each paw tested 5 times with at least 2 min break in between
Lowest and highest reflex latency scores of each paw were discarded
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