SNP Genotyping of SPR Gene

Genomic DNA was extracted from ethylene diamine tetraacetic acid-treated peripheral blood using the TIANamp Blood DNA Kit (Tiangen Biotech, Beijing, People’s Republic of China). In total, 1,885 individuals were genotyped for two SNPs (rs1876487 and rs2421095) located in the SPR gene using the SNaPshot technique (Thermo Fisher Scientific, Waltham, MA, USA) as described previously.19 (link) The polymerase chain reaction primers used for rs1876487 were 5′-AGGGCTGGAACTGGGAGGAAAT-3′ (forward primer) and 5′-TGGTTCCCTTGGGATCTGGTTC-3′ (reverse primer), and the primers used for rs2421095 were 5′-CCTCCAAGTTGTTTCTTCCTTAGAGTTG-3′ and 5′-TTAGCCCARTTCCCCACAGG-3′.