C57BL/6 mice were intraperitoneally (ip) injected with Mito-TEMPO (20 mg/kg, Sigma–Aldrich, SML0737) or Mdivi-1(25 mg/kg, GLPBIO, GC10200), and after 1 h, the mice were injected with MSU suspension (3 mg in 100 μL PBS). After 6 h, the mice were sacrificed under CO2 anesthesia for extraction of peritoneal fluid. Peritoneal lavage fluid was collected to measure the IL-1β levels by ELISA and to assess lymphocyte recruitment by FCM using the leukocyte common antigen FITC-CD45 (BD BioScience, 553079) staining, Pacific blue-CD11b (Biolegend, USA, 101245) costaining with PE-F4/80 (BD BioScience, 565410), and APC-Ly-6G (Biolegend 127614), respectively.
The C57BL/6 mice were intraperitoneally injected with Mito-TEMPO (20 mg/kg, Sigma-Aldrich, SML0737) or Mdivi-1(25 mg/kg, GLPBIO, GC10200) and then injected the foot pad with MSU suspension (1 mg in 50 μL PBS). Twenty-four hours after injection with the MSU suspension, the swelling of the foot pad was measured with digital vernier caliper, and then the mice were sacrificed. Joint index evaluation was described previously [26 (link)].
The C57BL/6 mice were intraperitoneally injected with Mito-TEMPO (20 mg/kg, Sigma-Aldrich, SML0737) or Mdivi-1(25 mg/kg, GLPBIO, GC10200) and then injected the foot pad with MSU suspension (1 mg in 50 μL PBS). Twenty-four hours after injection with the MSU suspension, the swelling of the foot pad was measured with digital vernier caliper, and then the mice were sacrificed. Joint index evaluation was described previously [26 (link)].
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