Quantifying EPCs and SDF-1α in AVM

A total of 15 AVM patients were randomly selected for venous blood collection, along with 15 healthy controls. Flow cytometry technique was applied to analyze the percentage of EPCs in the total mononuclear cells as previously reported.11 (link) EPCs were defined as triple-positive signals for CD133, CD34, and KDR. Enzyme-linked immunosorbent assay (ELISA) was also employed to detect the level of SDF-1α in peripheral blood by using previously established protocols12 (link) and the human CXCL12/SDF-1α ELISA kit (R&D Systems, Minneapolis, MN, USA). A correlation analysis was then performed to identify the correlation between EPCs percentage and SDF-1α level in each individual sample.

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Wang L., Guo S., Zhang N., Tao Y., Zhang H., Qi T., Liang F, & Huang Z. (2015). The role of SDF-1/CXCR4 in the vasculogenesis and remodeling of cerebral arteriovenous malformation. Therapeutics and Clinical Risk Management, 11, 1337-1344.

Publication 2015

human CXCL12/SDF-1α ELISA kit

Cells Cxcl12 Enzyme linked immunosorbent assay Epcs Flow cytometry Human In blood Patients Venous

Corresponding Organization :

Other organizations : Sun Yat-sen University

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Variable analysis

independent variables

Venous blood collection from AVM patients and healthy controls

dependent variables

Percentage of EPCs in total mononuclear cells
Level of SDF-1α in peripheral blood

control variables

Flow cytometry technique used to analyze EPCs
ELISA used to detect SDF-1α level
Human CXCL12/SDF-1α ELISA kit (R&D Systems) used

controls

Healthy controls for comparison with AVM patients

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