C57BL/6N mice were purchased from CLEA JAPAN. Cd3ϵ-/- and OT-I-TCR transgenic mice have been described previously (31 (link)). All mice had a C57BL/6 genetic background. Male mice, aged 8 weeks and weighing 20–25 g, were used under cohousing conditions in specific pathogen-free facilities. All animal experiments were approved by the Animal Research Committee and Ethics Committee of Keio University School of Medicine.
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