Lipid Extraction and Mass Spectrometry Analysis
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Corresponding Organization :
Other organizations : University of Notre Dame, Faculdade Meridional, Weizmann Institute of Science
Protocol cited in 13 other protocols
Variable analysis
- Genotype (WT, Δsmt1, Δsmt1+SMT1, Δgcs1, Δgcs1+GCS1)
- Total lipids extracted
- Inositol-containing phospholipids and phosphatidylcholine
- Neutral lipids
- Mass spectrometry (MS) analysis of lipid species (α-OH-Δ4-Δ8,9methyl-GlcCer, α-OH-Δ4-Δ8-GlcCer, α-OH-Δ4-Δ8,9methyl-ceramide, α-OH-Δ4-Δ8-ceramide)
- Growth conditions (YPD medium, 30°C, 250 rpm, 20 hrs)
- Cell number (5 × 10^8 cells)
- Not specified
- Not specified
Annotations
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