Identifying eomesa Genes in Common Carp

Two copies of eomesa genes were identified in the genome of common carp. The eomesa1 and eomesa2 cDNA sequences of goldfish (Carassius auratus), Japanese silver crucian carp (Carassius auratus langsdorfii) and common carp downloaded from GenBank were used to identify and confirm the eomesa gene sequences and structures in the common carp genome (http://www.fishbrowser.org/database/Commoncarp_genome) using BLAST program (S1 Table).
The genomic sequences of common carp eomesa1 and eomesa2 were compared. Regions with low similarity and uniqueness to each gene were selected to design specific primer pairs to amplify eomesa1 and eomesa2 separately (Table 1, S1 Fig). The amplified fragments were then sequenced with the Sanger method. The sequencing results showed that these primers could be used to specifically amplify eomesa1 and eomesa2 of color common carp.