As for the collection of blood samples, antecubital venous blood samples (approximately 10 mL) were obtained from all patients included in this study on the morning of the operation day. The blood samples collected from each participant were put into ethylenediamine tetraacetic (EDTA) tubes with added aprotinin and taken to the laboratory within 30 min. The blood samples taken for the measurement of afamin level were centrifuged in Nüve (Nüve Sanayi Malzemeleri İmalat ve Ticaret A.Ş, Ankara, Turkey) NF800 centrifuge devices at 4000 rpm for 10 min, and following the separation of the serum part, the serum samples were transferred to Eppendorf tubes and stored at −80 °C until the study day. Insulin (fasting), HgbA1c, TSH, T4, total cholesterol, triglyceride, LDL, HDL, and VLDL serum values within the scope of this study were taken during the preoperative evaluation one week before the surgery. Antecubital venous samples (approximately 25 mL) were collected at 6 months postoperatively while the patients had an empty stomach. The serum part of the blood samples collected for the measurement of the afamin level was separated and stored at −80 °C. Insulin (fasting), HgbA1c, TSH, T4, total cholesterol, triglyceride, LDL, HDL, and VLDL values were evaluated routinely in the hospital laboratory. Afamin levels were measured by the enzyme-linked immunosorbent assay (ELISA) method. Afamin was stored at room temperature for about half an hour with Human Afamin ELISA kits manufactured by AFG Scientific (catalog number EK 714650, AFG Bioscience LLC, USA). After washing with an Awareness brand Stat Fax 2600 device, afamin levels were evaluated with an Awareness brand ChroMate 4300 device and immunoassay 450 wavelength method. The values were measured according to the manufacturer’s instructions.
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