Low acyl GG (Gelzan), methacrylic anhydride (MA, purity ≥ 94%), sodium hydroxide (NaOH, reagent grade, 98%), 2-hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, gas chromatography area ≥ 98%), and absolute ethanol (EtOH, purity ≥ 99.9%) were obtained from Sigma Aldrich (Milan, Italy) and were used for GGMA synthesis.
Calcium nitrate tetrahydrate (Ca(NO3)24H2O, ACS reagent, 99%), diammonium hydrogen phosphate ((NH4)2HPO4, ACS reagent Ph Eur.), ammonium hydroxide (NH4OH, ACS reagent, 28.0–30.0% NH3 basis), phosphate-buffered saline (PBS), were from Sigma Aldrich (Milan, Italy) and were used for HAp synthesis.
Lactic acid (C3H6O3), hydrochloric acid (HCl, ACS reagent, 37%), were from Sigma Aldrich (Singapore) and were used for BSF-Eumel extraction.
Calcium chloride (CaCl2, ≥ 96.0%) was from Sigma Aldrich (Milan, Italy) and was employed for 3D-hydrogel preparation. Fresh deionized (diH2O) water (for chromatography LC-MS Grade, conductance at 25 °C ≤ 1 µS/cm, Sigma Aldrich, Milan, Italy) was used throughout the chemical synthesis, while distilled water (dH2O) was employed for purification steps and characterization studies.
Murine osteoblast-like cell line (7F2, CRL-12557) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and adopted for scaffold in vitro validation studies. Dulbecco’s Modified Eagle Medium high glucose with and without Phenol Red (DMEM-HG) were from Gibco by Thermo Fisher Scientific (Monza, Italy). Dimethyl sulfoxide (DMSO, purity ≥ 99.9%), formalin solution (neutral buffered 10%), fetal bovine serum (FBS), antibiotic/antimycotic solution (100×), L-glutamine (200 mM), trypsin-EDTA solution (Tryp-EDTA, 1×) and 4′,6-diaminidin-2-fenilindolo (DAPI) were all from Sigma Aldrich (Milan, Italy) and were used for cell maintenance and in vitro studies. Red Cell Tracker Red CMTPX was from Thermo Fisher Scientific (Milan, Italy) and was used for fluorescence microscopy studies.
Calcium nitrate tetrahydrate (Ca(NO3)24H2O, ACS reagent, 99%), diammonium hydrogen phosphate ((NH4)2HPO4, ACS reagent Ph Eur.), ammonium hydroxide (NH4OH, ACS reagent, 28.0–30.0% NH3 basis), phosphate-buffered saline (PBS), were from Sigma Aldrich (Milan, Italy) and were used for HAp synthesis.
Lactic acid (C3H6O3), hydrochloric acid (HCl, ACS reagent, 37%), were from Sigma Aldrich (Singapore) and were used for BSF-Eumel extraction.
Calcium chloride (CaCl2, ≥ 96.0%) was from Sigma Aldrich (Milan, Italy) and was employed for 3D-hydrogel preparation. Fresh deionized (diH2O) water (for chromatography LC-MS Grade, conductance at 25 °C ≤ 1 µS/cm, Sigma Aldrich, Milan, Italy) was used throughout the chemical synthesis, while distilled water (dH2O) was employed for purification steps and characterization studies.
Murine osteoblast-like cell line (7F2, CRL-12557) was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and adopted for scaffold in vitro validation studies. Dulbecco’s Modified Eagle Medium high glucose with and without Phenol Red (DMEM-HG) were from Gibco by Thermo Fisher Scientific (Monza, Italy). Dimethyl sulfoxide (DMSO, purity ≥ 99.9%), formalin solution (neutral buffered 10%), fetal bovine serum (FBS), antibiotic/antimycotic solution (100×), L-glutamine (200 mM), trypsin-EDTA solution (Tryp-EDTA, 1×) and 4′,6-diaminidin-2-fenilindolo (DAPI) were all from Sigma Aldrich (Milan, Italy) and were used for cell maintenance and in vitro studies. Red Cell Tracker Red CMTPX was from Thermo Fisher Scientific (Milan, Italy) and was used for fluorescence microscopy studies.
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