Protein Crystal Screening with Mosquito Nanolitre Handler

MRC crystallization plates (Swissci) containing MORPHEUS (85 µl in the main wells) were prepared on a Mosquito (TTP labtech) or ScreenMaker (Innovadyne) nanolitre liquid handler. Our standard setup for initial screens is to mix equal-volume aliquots of the protein and condition at 297 K, with a 200 nl final volume of drops, and to store the plates at 292 K. Final assessments were made after one week by manual inspection using a high-powered Leica MX-12 stereomicroscope. A drop was considered a crystallization hit when it contained protein crystals larger than 20 µm, so that they could be mounted in a cryoloop for X-ray diffraction.

Free full text: Click here

, & Gorrec F. (2009). The MORPHEUS protein crystallization screen. Journal of Applied Crystallography, 42(Pt 6), 1035-1042.

Publication 2009

Crystallization Mosquito Protein Standard setup X ray diffraction

Corresponding Organization : MRC Laboratory of Molecular Biology

Top 5 similar protocols

Protocol cited in 53 other protocols

Variable analysis

independent variables

Liquid handling method (Mosquito or ScreenMaker)
Final volume of drops (200 nl)
Protein and condition mixing ratio (equal-volume aliquots)
Incubation temperature (297 K)

dependent variables

Presence and size of protein crystals (larger than 20 µm)

control variables

Plate type (MRC crystallization plates, Swissci)
Buffer composition (MORPHEUS, 85 µl in the main wells)
Storage temperature (292 K)
Assessment method (manual inspection using a Leica MX-12 stereomicroscope)

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!