Flow Cytometry Standardized Leukemia Panel

Flow cytometry staining was performed with the standardized EuroFlow ALOT (acute leukemia orientation tube) antibody panel (CD45-OC515, CD3-Pacific Blue, CyMPO-FITC, CyCD79a-PE, CD34-PerCP/Cy5.5, CD19-PE/Cy7, CD7-APC, SmCD3, APC/C750) and further tubes 1-3,^{27 (link)} samples were acquired by using a FACSCanto II (Becton Dickinson), and data were analyzed by using Infinicyt 2.0 (Cytognos).

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Juárez-Avendaño G., Luna-Silva N.C., Chargoy-Vivaldo E., Juárez-Martínez L.A., Martínez-Rangel M.N., Zárate-Ortiz N., Martínez-Valencia E., López-Martínez B., Pelayo R, & Balandrán J.C. (2020). Poor Prognosis Biomolecular Factors Are Highly Frequent in Childhood Acute Leukemias From Oaxaca, Mexico. Technology in Cancer Research & Treatment, 19, 1533033820928436.

Publication 2020

FACSCanto II Infinicyt 2.0

Antibody Cy5 5 Fitc Flow cytometry Leukemia

Corresponding Organization : Hospital Juárez de México

Other organizations : Institute for Social Security and Services for State Workers, Hospital General de México, Hospital Infantil de México Federico Gómez, Mexican Social Security Institute

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Variable analysis

independent variables

Flow cytometry staining protocol

dependent variables

Analyzed data

control variables

Standardized EuroFlow ALOT (acute leukemia orientation tube) antibody panel
FACSCanto II (Becton Dickinson) flow cytometer
Infinicyt 2.0 (Cytognos) software for data analysis

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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