DPPH free radical scavenging activity of the aqueous plant extracts was carried out according to a method described by Zheleva-Dimitrova [22 (link)] with slight modifications. Briefly, 10 μL of the different concentrations of the aqueous plant extracts was reacted with 190 μL of DPPH solution (0.00625 g DPPH in 50 mL methanol) and the absorbance of the samples was determined after 30 min using a Multiskan Spectrum plate reader (Thermo Fisher Scientific, USA) at 517 nm. Free radical scavenging activity of the samples was expressed according to the equation below:
where A0 is the absorbance of DPPH• in solution without an antioxidant and A is the absorbance of DPPH• in the presence of an antioxidant. IC50 value (concentration of sample where absorbance of DPPH decreases 50% with respect to absorbance of blank) of the sample was determined. All determinations were done in triplicates.
where A0 is the absorbance of DPPH• in solution without an antioxidant and A is the absorbance of DPPH• in the presence of an antioxidant. IC50 value (concentration of sample where absorbance of DPPH decreases 50% with respect to absorbance of blank) of the sample was determined. All determinations were done in triplicates.
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