Circulating white blood cell counts after 6 weeks of E2 supplementation (vs. age-matched controls) were determined using a Hemavet 950DS (Drew Scientific, Miami Lakes, FL). E2 levels in serum collected 2 or 6 weeks post pellet placement, and stored at −80°C prior to assay, were assayed by the University of Virginia Center for Research in Reproduction Ligand Assay and Analysis Core using a commercially available mouse/rat estradiol ELISA (Calbiotech, El Cajon, CA)[38 (link)]. Markers of bone formation (rat/mouse P1NP) or resorption (mouse TRAcP 5b) were assayed in fasting serum collected 2 weeks after start of E2 supplementation (vs. age-matched controls) using commercially available ELISAs (Immunodiagnostic Systems, United Kingdom) [22 (link),25 (link)].
Protocol detail
Estrogen Supplementation: Immune and Bone Effects
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : University of Arizona
Top 5 similar protocols
Protocol cited in 2 other protocols
Variable analysis
independent variables
- E2 supplementation
- Circulating white blood cell counts
- E2 levels in serum
- Markers of bone formation (rat/mouse P1NP)
- Markers of bone resorption (mouse TRAcP 5b)
- Age-matched controls
- None specified
- None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!