Total RNA from frozen mouse colon tissue was isolated using TRI Reagent (Molecular Research Center, Inc., Cincinnati, OH, USA). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using the StepOnePlus detection system (Applied Biosystems, Foster City, CA, USA) and the TaqMan One-Step RT-PCR kit containing AmpliTaq Gold® DNA polymerase [23 (link)]. Specific primers and probes were obtained from Applied Biosystems. Fold changes in qPCR expression were calculated by the 2(−ΔΔCT) method [24 (link)].
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Quantitative RT-PCR from Mouse Colon Tissue
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Corresponding Organization :
Other organizations : Lovelace Respiratory Research Institute, Florida International University, Sylvester Comprehensive Cancer Center, University of Miami, University of New Mexico
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Variable analysis
independent variables
- Independent variables not explicitly mentioned.
- Fold changes in qPCR expression
- Control variables not explicitly mentioned.
- Positive control: Not specified.
- Negative control: Not specified.
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