Intravitreal Injection of P2X7R Antagonist

Injections were performed under a microscope with a micropipette connected to a microsyringe (Drummond Scientific Co., Broomall, PA, USA) as described (Hu et al., 2010 (link)). P2X7R antagonist BBG (0.8%) was dissolved in sterile saline and injected 5–7 days before IOP elevation. The glass pipette filled with drug was passed through the sclera at a point approximately 1 mm from the limbus into the vitreous cavity. The total volume injected was 5 μl over a 30 s time period.

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Lim J.C., Lu W., Beckel J.M, & Mitchell C.H. (2016). Neuronal Release of Cytokine IL-3 Triggered by Mechanosensitive Autostimulation of the P2X7 Receptor Is Neuroprotective. Frontiers in Cellular Neuroscience, 10, 270.

Publication 2016

micropipette

Cavity Drug Microscope Saline Sclera Sterile

Corresponding Organization : University of Pennsylvania

Other organizations : University of Pittsburgh

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Variable analysis

independent variables

P2X7R antagonist BBG (0.8%) injection

dependent variables

IOP elevation

control variables

Microsyringe used for injections
Glass pipette used for injections
Injection volume of 5 μl over 30 s
Injection site approximately 1 mm from the limbus into the vitreous cavity

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